Enzymology and Catalytic Mechanism Essay Example for Free

Enzymology and Catalytic Mechanism Essay Many diseases and disorders are caused by defects in the molecular level of cells and not just having to do with nutrition or other extrinsic factors ,in this report ,we will specifically look at enzymes and their key role in energy production and the cycles and series of activities that make it possible for the human to function properly . Enzymes have four basic characteristics .They do not make a reaction occur that would not occur on its own, they just enhance and make it happen quicker. The enzyme will not be changed by the reaction, it may be changed during the process, but the end product will leave the enzyme as it was when it started. Enyzmes can be used repeatedly. Enyzmes have specific substances in our bodies, so if enzymes don’t work properly, metabolism will not work properly. Enzymes do their work as catalyst by speeding up the process to improve performance. They lessen the activation energy making producing products easier .Enzymes act on specific substances in our bodies. The enzymes will fit into the substance or substrate like a lock and key .Once it is with its certain substrate, it acts like a key and it unlocks the substrate and the substrate can do what it needs to do and then the enzyme will releases from it and is not changed ,then it moves on to another substance. Heredity Fructose Intolerance is a condition that can be passed down through families ,in HFI,two copies of an abnormal gene are present ,thus making it an auto recessive diso rder, and frequently it is seen in person without a history of the disorder . HFI is a condition that affects fructose metabolism ,basically the amount of Aldolase B’s substrate ,F1P ,changes when Aldolase B is deficient or doesn’t work at all. People that have this disorder do not have any symptoms if they do not ingest any  fructose ,whether orally or by IV .F1P is a product of fructokinase and substrate for Aldolase B ,when Aldolase B is not available ,we start to see symptoms of HFI .Aldolase B is only seen in the kidney,liver,and small intestines. An excellent way to see enzymatic roles in our bodies is by studying how Fructose is metabolized in our liver .It is a multiply step, complex process .The first step is the conversion of fructose to fructose 1-phosphate by fructokinase ,which causes the fructose to become trapped in the liver .Then,Fructose-1-phosphate undergoes the chemical reaction called hydrolysis by fructose-1-phosphate aldolase (aldolase B) to form dihydroxyacetone phosphate (DHAP) and glyceraldehyde. This usage of fructose leads to either glycolysis for the production of ATP or gluconeogenesis.Sanders, J. (2013) When fructose is taken into the body it is phosphorylated by fructokinase to make fructose 1 –phosphate , Aldolase B then impels F1P breakdown into glyceraldehyde and DHAP,that are used to go on to make ATP through glycolysis or to be used for storage through gluconeogenesis(glycolysis in reverse) .Fructose 1 Phosphate is a product and substrate but also a signal and it send out an alert for high blood sugar and will send signals to tell glucokinase to come out of the nucleus and into the cytoplasm and lower blood sugar by sending out glucose to the body. Our bodies usually only have fructose in the blood if we have an increase amount of glucose or high blood sugar ,this means we have elevated F1P levels and that is when glucokinase needs to be released .When F1P is low and our blood sugar is low and our blood glucose is low ,glucokinase will go into the nucleus. When the liver senses hypoglycemia, it produces glucose to be sent to liver cells .Glycolysis reverses to gluconeogenesis to produce glucose to be released in the blood. With HFI,if there is an abundance of F1P because there is no Aldolase B,then our blood sugar is low and the liver will not be picking up the phosphate and it is not being used glycogen breakdown will be slowed down and not much glucose will get produced and glucose will not be getting released, that’s when you see symptoms of hypoglycemia .Also, symptoms of liver damage will result because there is no phosphate available for the ATP synthase ,because it is bound up ,the cells in the liver will die without the ATP production .If a person continues to ingest and not be able to metabolize the fructose a person can die .Symptoms of HFI are hypoglycemia,jaundic,nausea/vomiting, renal or  liver damage or failure. .When bodies are overworked; our breathing will speed up as we try to get more oxygen delivered to our body cells. Most of our energy is made through aerobic methods; however, some situations need an accelerated more energy than our bodies can effectively make. That is when our bodies will cause muscles to generate energy through glycolysis. During glycolysis, glucose breaks down into pyruvate. If our bodies have enough oxygen; pyruvate is goes into an aerobic pathway to be broken down and used for energy. When we don’t have enough oxygen, our body will temporarily change pyruvate into lactate and it will be sent through a cycle to change it to glucose. If there are high lactate levels in the muscle cells there will be a high level of acidity and other metabolites will be disrupted .The high lactate levels do prevent permanent muscle damage when muscles are exerted by slowing the primary systems needed for muscle contraction, but when the body rest, oxygen becomes available and the lactate changes back to pyruvate, allowing continued aerobic metabolism and energy for when a persons the body is recovering from an extreme physical exertion. In glycolysis, 2 ATP are produced to turn glucose into lactate, but in gluconeogenous, it takes 6 ATP to lactate back into glucose. In our muscles glucose can combine with oxygen to generate energy. If there is not any oxygen, the anaerobic breakdown of glucose is achieved through glycolysis; however, ATP and Lactate are present through this process. In the normal presence of O2, glycolysis in muscle cells produces two units of ATP and two units of pyruvate. The two compounds provide the energy that enables a cell to perpetuate breathing through a series of chemical reactions called the Krebs cycle, also called the citric acid or tricarboxylic acid cycle. Oxidation pulls a carbon atom and 2 hydrogen atoms, water and carbon dioxide, out of the equation. Higa, M. (2015) In the absence of oxygen, enzymes can break down the glucose carbohydrate by fermentation. Dehydrogenase enzyme in muscle cells converts it into lactate and the amino acid alanine. The liver filters the lactate out of blood to reverse engineer it to pyruvate and then into glucose. While two ATP molecules are produced by glycolysis in the muscles, it costs the liver six ATP molecules to feed the cycle by gluconeogenesis. If the Cori Cycle occurred in one cell, then glucose to lactate would make 2 ATP, but the lactate to glucose process would cost 6 ATP, this is a net loss of 4 ATP, if the cycle continued over  and over then it would be a futile cycle. If there is any defect with the enzymes in the citric acid cycle ,the cycle will not continue to function as it should, every step has a specific role and a lot of the time the enzymes and products relay on one to function properly for the next step to occur, an example would be :if citrate synthase did not occur or if there was a defect ,then Acetyl-CoA would not happen, nor would CoA,therefore ,the following steps in the CAC would not happen as they should and since it is a cycle, each part has to function properly. The biggest problem with a break in the CAC is that the ATP production is not possible because CAC products are sent to the Electron Transport Chain for Oxidative phosphorylation. During the ETC ,products from Citric Acid Cycle are sent to the matrix of the mitochondria ,the workhouse of the eukaryotic cells ,and it is there ,that NADH and FADH2 are sent to a series of 4 complexes, first to complex 1 and then to complex 2 and then then both of them send their electrons to a co-enzyme ,named Q10, and then from there these products transfer to complex 3 and then to co-enzyme Cytochrome C and then complex 4 ,at this point these the electrons are given for oxygen to make water . This is called Aerobic Respirations. Oxygen takes the electrons and makes water and then the hydrogen ions are pumped out across the intra membranes space. The complex I, III, and pushes these hydrogen ions out .The next complex are called ATP synthase. These hydrogen ions move through this complex and they have energy and they take ADP and phosphate and make it in ATP. Sanders, J. (2013) If any enzyme in the CAC stops working the amount of ATP is affected, example would be if citrate synthase is not working properly then citrate would not be made, also CoA nor would Acetyl- CoA, which is the molecule that enters the CAC.Subsequently the entire cycle is disrupted and then `the ETC will not have access to the products required for ATP production. Sources: Sanders, J. (2013).Electron Transport Chain Retrieved from http://wgu.hosted.panopto.com/Panopto/Pages/Viewer/Default.aspx?id=9ec716ab-3610-47a4-bb37-41b4805935d6 Gilbert, S. (2014).Enzymes Retrieved from http://www.biologyreference.com/Dn-Ep/Enzymes.html Sanders, J. (2013) Aldolase B and Hereditary Fructose Intolerance. Retrieved from http://wgu.hosted.panopto.com/Panopto/Pages/Viewer/Default.aspx?id=ce81226c-d293-4232-997e-fb2957530367 Higa, M. (2015) What is the Cori Cycle .Retrieved from http://www.wisegeek.com/what-is-the-cori-cycle.html

Walmart Essay -- essays research papers

By exploiting core competencies firms can develop value creating strategies which are superior to their competitors. Wal-Mart are experts at using there core competencies to become superior to their competitors. There are three resources which allow a company to create a core competency. Tangible resources consist of assets that can be seen, financial resources (borrowing capacity), physical resources, organizational structure and technology. Wal-Mart is a huge and very powerful company and therefore uses its ability of lending to become a core competency. Intangible resources are characterized by human resources, resource for innovation, and reputation. This category is where Wal-Mart excels against all its competitors. Knowledge, training and corporate culture possessed by employees may be one of the most significant sources of core competencies and competitive advantages throughout the business world. This is due to being very hard to copy or substitute for. Brand equity consists of brand name and maintaining brand equity. Wal-Mart are masters at using this resource as an advantage over their competitor are Wal-Marts emphasis is on its image of everyday low prices and high quality goods when marketing. Wal-Mart uses many different channels when marketing itself. It uses television, radio, monthly circulars, weekly newspapers and many more channels. Each one of these channels can be used in an unique way to emphasize Wal-Mart’s position of selling quality products at low prices. Radio usually grabs the audience’s attention by promoting products which are experiencing high demand. Both of these channels are made stronger by the use of newspapers adverts and monthly circulars. In these marketing channels deeply discounted items are highlighted to the potential competitors and these items help lure the customers into the stores. The idea of having â€Å"quality for less† is a good marketing plan because it gets people into the store. It also offers a competitive advantage over the competitors because they can not financially match Wal-Mart prices. This is due to Wal-Mar t having better use of financial resources, technology and physical resources. By censoring some products Wal-Mart are trying to market themselves as a company with good values. They use this to attract families. Unfortunately this plan has negative and positive effects. A negative effect is the ef... ...eakness have been stated as an air of complacency or a loss of presentation and marketing in the stores. As a most firms grow they encounter problems with staying in the right direction. Wal-Mart must address this problem to continue to grow. Price deflation has been poorly by Wal-Mart and other retailers. â€Å"aiming to obtain sales increases, companies have bought more merchandise units of products that are experiencing price deflation, without allowing for the inelasticity of demand for most basic products† (lll). Basically this has reduced in markdowns which have resulted in reduced sales and gross margins. IFE Matrix The Internal factor Evaluation (IFE) matrix is used to summarize and evaluate the major strengths and weaknesses in the functional area of business. It also provides a basis for identifying and evaluating relationships between these areas. A weight is assigned to each factor with 0.0 being unimportant and 1.0 being all important. A rating is then assigned with 1 being minor weakness and 4 being a strength. Both of these are then multiplied together to get a weighted score. The closer the overall weighted score is to 4 the stronger the firm is. The average is 2.5.

Growth of Real Women

Education, voting, working were three things women could not do. Before the late 1800's women had mimited rights. As the years past by women slowly gained priviledges that men already had. Although women cant handle some of the tasks that men take on, women have many other advantages. In the early 1900's few women attended finishing schools called Female Seminaries. Later on the women who did attend state universities around 1863 had to live with being discrimintated against and being ridiculed. By the end of the 19th century female docotor and lawyers became more common. By The New Nationalism women got the right to vote in elections. Today womens rights have prgressed emensly. From not being able to tend school and get an education and not being able to have a job or vote women have gained all those rights. Now women are able to have higher paying jobs, full educations and vote for whoever they want. As apposed to long ago when they could only stay at home and cook. Now women are moving up in the ranks of the job industry. Some making more pay then males and being at higher rank. For example the Us has women comprise about a quarter of the UA facitlity. They are also moving up the rank to higher-paying staff posisions, more than 10 years ago. Although there are still more males, the women have increased tons over the last century. And as the studies show the women will still be rising in the future. From the past to the future women have gained more power over the years. From going to no rights at all women have gained as many rights as males have. Although some jobs are made for a man to work, women have the right to try and work there if that what they want to do. From reading the text book and reading the news article it is shown that we can make things happen. As women progressed over the years and fought for their rights as females, they won. I dont think anything will change in the future for women because they are already treated to the equal amount that men are. People across the US should know about this so they know they too can make a difference in the future.

Battle of Oriskany in the American Revolution

The Battle of Oriskany was fought August 6, 1777, during the American Revolution (1775-1783) and was part of Major General John Burgoynes Saratoga Campaign. Advancing through western New York, a British force led by Colonel Barry St. Leger laid siege to the American garrison at Fort Stanwix. Responding, the local militia, led by Brigadier General Nicholas Herkimer moved to aid the fort. On August 6, 1777, part of St. Legers force ambushed Herkimers column. The resulting Battle of Oriskany saw the Americans take heavy losses, but ultimately hold the battlefield. While they were prevented from relieving the fort, Herkimers men inflicted substantial casualties on St. Legers Native American allies, leading many to become disgruntled and leave the campaign, as well as provided an opportunity for the forts garrison to raid the British and Native American camps. Background In early 1777, Major General John Burgoyne proposed a plan for defeating the Americans. Believing that New England was the seat of the rebellion, he proposed severing the region from the other colonies by marching down the Lake Champlain-Hudson River corridor while a second force, led by Colonel Barry St. Leger, advanced east from Lake Ontario and through the Mohawk Valley. General John Burgoyne. Public Domain Rendezvousing at Albany, Burgoyne, and St. Leger would advance down the Hudson, while General Sir William Howes army advanced north from New York City. Though approved by Colonial Secretary Lord George Germain, Howes role in the plan was never clearly defined and issues of his seniority precluded Burgoyne from issuing him orders. Assembling a force of around 800 British and Hessians, as well as 800 Native American allies in Canada, St. Leger began moving up the St. Lawrence River and into Lake Ontario. Ascending the Oswego River, his men reached the Oneida Carry in early August. On August 2, St. Legers advance forces arrived at nearby Fort Stanwix. Garrisoned by American troops under Colonel Peter Gansevoort, the fort guarded the approaches to the Mohawk. Outnumbering Gansevoorts 750-man garrison, St. Leger surrounded the post and demanded its surrender. This was promptly refused by Gansevoort. As he lacked sufficient artillery for battering down the forts walls, St. Leger elected to lay siege (Map). Battle of Oriskany Conflict: American Revolution (1775-1783)Date: August 6, 1777Armies and Commanders:AmericansBrigadier General Nicholas Herkimerapprox. 800 menBritishSir John Johnsonapprox. 500-700 menCasualties:Americans: approx. 500 killed, wounded, and capturedBritish: 7 killed, 21 wounded/capturedNative Americans: approx. 60-70 killed and wounded American Response In mid-July, American leaders in Western New York first learned of a possible British attack into the region. Responding, the leader of Tryon Countys Committee of Safety, Brigadier General Nicholas Herkimer, issued a warning that the militia may be needed to block the enemy. On July 30, Herkimer received reports from friendly Oneidas that St. Legers column was within a few days march of Fort Stanwix. Upon receipt of this information, he immediately called out the countys militia. Gathering at Fort Dayton on the Mohawk River, the militia mustered around 800 men. This force included a group of Oneidas led by Han Yerry and Colonel Louis. Departing, Herkimers column reached the Oneida village of Oriska on August 5. Pausing for the night, Herkimer dispatched three messengers to Fort Stanwix. These were to inform Gansevoort of the militias approach and asked that receipt of the message be acknowledged by firing three cannons. Herkimer also requested that part of the forts garrison sortie to meet his command. It was his intention to remain in place until the signal was heard. As the next morning progressed, no signal was heard from the fort. Though Herkimer wished to remain at Oriska, his officers argued for resuming the advance. The discussions became increasingly heated and Herkimer was accused of being a coward and having Loyalist sympathies. Angered, and against his better judgment, Herkimer ordered the column to resume its march. Due to difficulty in penetrating the British lines, the messengers sent on the night of August 5 did not arrive until later the next day. The British Trap At Fort Stanwix, St. Leger learned of Herkimers approach on August 5. In an effort to prevent the Americans from relieving the fort, he ordered Sir John Johnson to take part of his Kings Royal Regiment of New York along with a force of rangers and 500 Seneca and Mohawks to attack the American column. Moving east, Johnson selected a deep ravine approximately six miles from the fort for an ambush. Deploying his Royal Regiment troops along the western exit, he placed the Rangers and Native Americans down the ravines sides. Once the Americans had entered the ravine, Johnsons men would attack while a Mohawk force, led by Joseph Brant, would circle around and strike the enemys rear. Mohawk leader Joseph Brant. Â  Public Domain A Bloody Day Around 10:00 AM, Herkimers force descended into the ravine. Though under orders to wait until the entire American column was in the ravine, a party of Native Americans attacked early. Catching the Americans by surprise, they killed Colonel Ebenezer Cox and wounded Herkimer in the leg with their opening volleys. Refusing to be taken to the rear, Herkimer was propped up under a tree and continued to direct his men. While the main body of the militia was in the ravine, those troops at the rear had not yet entered. These came under attack from Brant and many panicked and fled, though some did fight their way forward to join their comrades. Assailed on all sides, the militia took heavy losses and the battle soon degenerated into numerous small unit actions. Slowly regaining control of his forces, Herkimer began pulling back to the edge of the ravine and American resistance began to stiffen. Concerned about this, Johnson requested reinforcements from St. Leger. As the battle became a pitched affair, a heavy thunderstorm erupted which caused a one-hour break in the fighting. Resistance Stiffens Taking advantage of the lull, Herkimer tightened his lines and directed his men to fire in pairs with one firing and one loading. This was to ensure that a loaded weapon was always available should a Native American charge forward with a tomahawk or spear. As the weather cleared, Johnson resumed his attacks and, at the suggestion of Ranger leader John Butler, had some of his men reverse their jackets in an effort to make the Americans think a relief column was arriving from the fort. This bit of trickery failed as the Americans recognized their Loyalist neighbors in the ranks. Despite this, British forces were able to exert heavy pressure on Herkimers men until their Native American allies began to leave the field. This was largely due to both the unusually heavy losses sustained in their ranks as well as word arriving that American troops were looting their camp near the fort. Having received Herkimers message around 11:00 AM, Gansevoort had organized a force under Lieutenant Colonel Marinus Willett to sortie from the fort. Colonel Peter Gansevoort. Â  Public Domain Marching out, Willetts men attacked the Native American camps south of the fort and carried off plenty of supplies and personal belongings. They also raided Johnsons camp nearby and captured his correspondence. Abandoned at the ravine, Johnson found himself outnumbered and was forced to withdraw back to the siege lines at Fort Stanwix. Though Herkimers command was left in possession of the battlefield, it was too badly damaged to advance and retreated back to Fort Dayton. Aftermath In the wake of the Battle of Oriskany, both sides claimed victory. In the American camp, this was justified by the British retreat and Willetts looting of the enemy camps. For the British, they claimed success as the American column failed to reach Fort Stanwix. Casualties for the Battle of Oriskany are not known with certainty, though it is estimated that American forces may have sustained as many as 500 killed, wounded, and captured. Among the American losses was Herkimer who died on August 16 after having his leg amputated. Native American losses were approximately 60-70 killed and wounded, while British casualties numbered around 7 killed and 21 wounded or captured. Though traditionally seen as a clear American defeat, the Battle of Oriskany marked a turning point in St. Legers campaign in western New York. Angered by the losses taken at Oriskany, his Native American allies became increasingly disgruntled as they had not anticipated in taking part in large, pitched battles. Sensing their unhappiness, St. Leger demanded Gansevoorts surrender and stated that he could not guarantee the garrisons safety from being massacred by the Native Americans following a defeat in battle. This demand was immediately rejected by the American commander. In the wake of Herkimers defeat, Major General Philip Schuyler, commanding the main American army on the Hudson, dispatched Major General Benedict Arnold with around 900 men to Fort Stanwix. Reaching Fort Dayton, Arnold sent forward scouts to spread misinformation regarding the size of his force. Believing that a large American army was approaching, the bulk of St. Legers Native Americans departed and began fighting a civil war with the American-allied Oneidas. Unable to maintain the siege with his depleted forces, St. Leger was forced to begin retreating towards Lake Ontario on August 22. With the western advance checked, Burgoynes main thrust down the Hudson was defeated that fall at the Battle of Saratoga.